Autophagy has been linked to longevity in many species. but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter. we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/IGF-1 and glp-1/Notch mutants throughout adulthood. https://www.jmannino.com/great-choice-Disney-Princess-Cinderella-Curtsy-Art-Galaxy-S10-Clear-Case-p29207-mega-find/